Sequencing:Article Title: Local circuit amplification of spatial selectivity in the hippocampus
Article Snippet: pCAG-TVA800-WT-HA was assembled by in-fusion cloning using Addgene plasmid 15778 (gift from E. Callaway). .. Notably, the HA-coding sequence was included in the primer sequences to allow for seamless HA insertion during fusion of the TVA-PCR product into the linearized XhoI/NotI pCAG vector backbone. pCAGGS-N2c(G) was a gift from T. Jessel, Addgene plasmid 73481. pCAG-GCaMP7s was assembled by in-fusion cloning using Addgene plasmid 104487 (gift from D. Kim) for PCR amplification as a template and inserted into NotI/XhoI sites of a pCAG-vector backbone according to the manufacturer’s instructions. pCAG-bReaChES-mRuby3 and pCAG-ChRmine-mScarlet were constructed through infusion cloning using pAAV-CaMKIIa-bReaChES-TS-mRuby3 and pAAV-CaMKIIa-ChRmine-TS-mScarlet as a PCR template (gifts from K. Deisseroth) into XhoI/NotI sites of a pCAG-vector backbone. .. The pCAG-Cre-mRuby fusion was constructed in a two-step infusion cloning process using pCAG-Cre (Addgene plasmid 13775, gift from C. Cepko) and pCAG-mRuby3 (Addgene plasmid 107744, gift from R. Larsen) as PCR templates to result in a Cre-mRuby fusion single ORF.
Article Title: Local circuit amplification of spatial selectivity in the hippocampus.
Article Snippet: pCAG-TVA800-WT-HA was assembled by in-fusion cloning using Addgene plasmid 15778 (gift from E. Callaway). .. Notably, the HA-coding sequence was included in the primer sequences to allow for seamless HA insertion during fusion of the TVA-PCR product into the linearized XhoI/NotI pCAG vector backbone. pCAGGS-N2c(G) was a gift from T. Jessel, Addgene plasmid 73481. pCAG-GCaMP7s was assembled by in-fusion cloning using Addgene plasmid 104487 (gift from D. Kim) for PCR amplification as a template and inserted into NotI/XhoI sites of a pCAG-vector backbone according to the manufacturer’s instructions. pCAG-bReaChES-mRuby3 and pCAG-ChRmine-mScarlet were constructed through infusion cloning using pAAV-CaMKIIa-bReaChES-TS-mRuby3 and pAAV-CaMKIIa-ChRmine-TS-mScarlet as a PCR template (gifts from K. Deisseroth) into XhoI/NotI sites of a pCAG-vector backbone. .. The pCAG-Cre-mRuby fusion was constructed in a two-step infusion cloning process using pCAG-Cre (Addgene plasmid 13775, gift from C. Cepko) and pCAG-mRuby3 (Addgene plasmid 107744, gift from R. Larsen) as PCR templates to result in a Cre-mRuby fusion single ORF.
Plasmid Preparation:Article Title: Local circuit amplification of spatial selectivity in the hippocampus
Article Snippet: pCAG-TVA800-WT-HA was assembled by in-fusion cloning using Addgene plasmid 15778 (gift from E. Callaway). .. Notably, the HA-coding sequence was included in the primer sequences to allow for seamless HA insertion during fusion of the TVA-PCR product into the linearized XhoI/NotI pCAG vector backbone. pCAGGS-N2c(G) was a gift from T. Jessel, Addgene plasmid 73481. pCAG-GCaMP7s was assembled by in-fusion cloning using Addgene plasmid 104487 (gift from D. Kim) for PCR amplification as a template and inserted into NotI/XhoI sites of a pCAG-vector backbone according to the manufacturer’s instructions. pCAG-bReaChES-mRuby3 and pCAG-ChRmine-mScarlet were constructed through infusion cloning using pAAV-CaMKIIa-bReaChES-TS-mRuby3 and pAAV-CaMKIIa-ChRmine-TS-mScarlet as a PCR template (gifts from K. Deisseroth) into XhoI/NotI sites of a pCAG-vector backbone. .. The pCAG-Cre-mRuby fusion was constructed in a two-step infusion cloning process using pCAG-Cre (Addgene plasmid 13775, gift from C. Cepko) and pCAG-mRuby3 (Addgene plasmid 107744, gift from R. Larsen) as PCR templates to result in a Cre-mRuby fusion single ORF.
Article Title: Local circuit amplification of spatial selectivity in the hippocampus.
Article Snippet: pCAG-TVA800-WT-HA was assembled by in-fusion cloning using Addgene plasmid 15778 (gift from E. Callaway). .. Notably, the HA-coding sequence was included in the primer sequences to allow for seamless HA insertion during fusion of the TVA-PCR product into the linearized XhoI/NotI pCAG vector backbone. pCAGGS-N2c(G) was a gift from T. Jessel, Addgene plasmid 73481. pCAG-GCaMP7s was assembled by in-fusion cloning using Addgene plasmid 104487 (gift from D. Kim) for PCR amplification as a template and inserted into NotI/XhoI sites of a pCAG-vector backbone according to the manufacturer’s instructions. pCAG-bReaChES-mRuby3 and pCAG-ChRmine-mScarlet were constructed through infusion cloning using pAAV-CaMKIIa-bReaChES-TS-mRuby3 and pAAV-CaMKIIa-ChRmine-TS-mScarlet as a PCR template (gifts from K. Deisseroth) into XhoI/NotI sites of a pCAG-vector backbone. .. The pCAG-Cre-mRuby fusion was constructed in a two-step infusion cloning process using pCAG-Cre (Addgene plasmid 13775, gift from C. Cepko) and pCAG-mRuby3 (Addgene plasmid 107744, gift from R. Larsen) as PCR templates to result in a Cre-mRuby fusion single ORF.
Cloning:Article Title: Local circuit amplification of spatial selectivity in the hippocampus
Article Snippet: pCAG-TVA800-WT-HA was assembled by in-fusion cloning using Addgene plasmid 15778 (gift from E. Callaway). .. Notably, the HA-coding sequence was included in the primer sequences to allow for seamless HA insertion during fusion of the TVA-PCR product into the linearized XhoI/NotI pCAG vector backbone. pCAGGS-N2c(G) was a gift from T. Jessel, Addgene plasmid 73481. pCAG-GCaMP7s was assembled by in-fusion cloning using Addgene plasmid 104487 (gift from D. Kim) for PCR amplification as a template and inserted into NotI/XhoI sites of a pCAG-vector backbone according to the manufacturer’s instructions. pCAG-bReaChES-mRuby3 and pCAG-ChRmine-mScarlet were constructed through infusion cloning using pAAV-CaMKIIa-bReaChES-TS-mRuby3 and pAAV-CaMKIIa-ChRmine-TS-mScarlet as a PCR template (gifts from K. Deisseroth) into XhoI/NotI sites of a pCAG-vector backbone. .. The pCAG-Cre-mRuby fusion was constructed in a two-step infusion cloning process using pCAG-Cre (Addgene plasmid 13775, gift from C. Cepko) and pCAG-mRuby3 (Addgene plasmid 107744, gift from R. Larsen) as PCR templates to result in a Cre-mRuby fusion single ORF.
Article Title: Local circuit amplification of spatial selectivity in the hippocampus.
Article Snippet: pCAG-TVA800-WT-HA was assembled by in-fusion cloning using Addgene plasmid 15778 (gift from E. Callaway). .. Notably, the HA-coding sequence was included in the primer sequences to allow for seamless HA insertion during fusion of the TVA-PCR product into the linearized XhoI/NotI pCAG vector backbone. pCAGGS-N2c(G) was a gift from T. Jessel, Addgene plasmid 73481. pCAG-GCaMP7s was assembled by in-fusion cloning using Addgene plasmid 104487 (gift from D. Kim) for PCR amplification as a template and inserted into NotI/XhoI sites of a pCAG-vector backbone according to the manufacturer’s instructions. pCAG-bReaChES-mRuby3 and pCAG-ChRmine-mScarlet were constructed through infusion cloning using pAAV-CaMKIIa-bReaChES-TS-mRuby3 and pAAV-CaMKIIa-ChRmine-TS-mScarlet as a PCR template (gifts from K. Deisseroth) into XhoI/NotI sites of a pCAG-vector backbone. .. The pCAG-Cre-mRuby fusion was constructed in a two-step infusion cloning process using pCAG-Cre (Addgene plasmid 13775, gift from C. Cepko) and pCAG-mRuby3 (Addgene plasmid 107744, gift from R. Larsen) as PCR templates to result in a Cre-mRuby fusion single ORF.
Polymerase Chain Reaction:Article Title: Local circuit amplification of spatial selectivity in the hippocampus
Article Snippet: pCAG-TVA800-WT-HA was assembled by in-fusion cloning using Addgene plasmid 15778 (gift from E. Callaway). .. Notably, the HA-coding sequence was included in the primer sequences to allow for seamless HA insertion during fusion of the TVA-PCR product into the linearized XhoI/NotI pCAG vector backbone. pCAGGS-N2c(G) was a gift from T. Jessel, Addgene plasmid 73481. pCAG-GCaMP7s was assembled by in-fusion cloning using Addgene plasmid 104487 (gift from D. Kim) for PCR amplification as a template and inserted into NotI/XhoI sites of a pCAG-vector backbone according to the manufacturer’s instructions. pCAG-bReaChES-mRuby3 and pCAG-ChRmine-mScarlet were constructed through infusion cloning using pAAV-CaMKIIa-bReaChES-TS-mRuby3 and pAAV-CaMKIIa-ChRmine-TS-mScarlet as a PCR template (gifts from K. Deisseroth) into XhoI/NotI sites of a pCAG-vector backbone. .. The pCAG-Cre-mRuby fusion was constructed in a two-step infusion cloning process using pCAG-Cre (Addgene plasmid 13775, gift from C. Cepko) and pCAG-mRuby3 (Addgene plasmid 107744, gift from R. Larsen) as PCR templates to result in a Cre-mRuby fusion single ORF.
Article Title: Local circuit amplification of spatial selectivity in the hippocampus.
Article Snippet: pCAG-TVA800-WT-HA was assembled by in-fusion cloning using Addgene plasmid 15778 (gift from E. Callaway). .. Notably, the HA-coding sequence was included in the primer sequences to allow for seamless HA insertion during fusion of the TVA-PCR product into the linearized XhoI/NotI pCAG vector backbone. pCAGGS-N2c(G) was a gift from T. Jessel, Addgene plasmid 73481. pCAG-GCaMP7s was assembled by in-fusion cloning using Addgene plasmid 104487 (gift from D. Kim) for PCR amplification as a template and inserted into NotI/XhoI sites of a pCAG-vector backbone according to the manufacturer’s instructions. pCAG-bReaChES-mRuby3 and pCAG-ChRmine-mScarlet were constructed through infusion cloning using pAAV-CaMKIIa-bReaChES-TS-mRuby3 and pAAV-CaMKIIa-ChRmine-TS-mScarlet as a PCR template (gifts from K. Deisseroth) into XhoI/NotI sites of a pCAG-vector backbone. .. The pCAG-Cre-mRuby fusion was constructed in a two-step infusion cloning process using pCAG-Cre (Addgene plasmid 13775, gift from C. Cepko) and pCAG-mRuby3 (Addgene plasmid 107744, gift from R. Larsen) as PCR templates to result in a Cre-mRuby fusion single ORF.
Amplification:Article Title: Local circuit amplification of spatial selectivity in the hippocampus
Article Snippet: pCAG-TVA800-WT-HA was assembled by in-fusion cloning using Addgene plasmid 15778 (gift from E. Callaway). .. Notably, the HA-coding sequence was included in the primer sequences to allow for seamless HA insertion during fusion of the TVA-PCR product into the linearized XhoI/NotI pCAG vector backbone. pCAGGS-N2c(G) was a gift from T. Jessel, Addgene plasmid 73481. pCAG-GCaMP7s was assembled by in-fusion cloning using Addgene plasmid 104487 (gift from D. Kim) for PCR amplification as a template and inserted into NotI/XhoI sites of a pCAG-vector backbone according to the manufacturer’s instructions. pCAG-bReaChES-mRuby3 and pCAG-ChRmine-mScarlet were constructed through infusion cloning using pAAV-CaMKIIa-bReaChES-TS-mRuby3 and pAAV-CaMKIIa-ChRmine-TS-mScarlet as a PCR template (gifts from K. Deisseroth) into XhoI/NotI sites of a pCAG-vector backbone. .. The pCAG-Cre-mRuby fusion was constructed in a two-step infusion cloning process using pCAG-Cre (Addgene plasmid 13775, gift from C. Cepko) and pCAG-mRuby3 (Addgene plasmid 107744, gift from R. Larsen) as PCR templates to result in a Cre-mRuby fusion single ORF.
Article Title: Local circuit amplification of spatial selectivity in the hippocampus.
Article Snippet: pCAG-TVA800-WT-HA was assembled by in-fusion cloning using Addgene plasmid 15778 (gift from E. Callaway). .. Notably, the HA-coding sequence was included in the primer sequences to allow for seamless HA insertion during fusion of the TVA-PCR product into the linearized XhoI/NotI pCAG vector backbone. pCAGGS-N2c(G) was a gift from T. Jessel, Addgene plasmid 73481. pCAG-GCaMP7s was assembled by in-fusion cloning using Addgene plasmid 104487 (gift from D. Kim) for PCR amplification as a template and inserted into NotI/XhoI sites of a pCAG-vector backbone according to the manufacturer’s instructions. pCAG-bReaChES-mRuby3 and pCAG-ChRmine-mScarlet were constructed through infusion cloning using pAAV-CaMKIIa-bReaChES-TS-mRuby3 and pAAV-CaMKIIa-ChRmine-TS-mScarlet as a PCR template (gifts from K. Deisseroth) into XhoI/NotI sites of a pCAG-vector backbone. .. The pCAG-Cre-mRuby fusion was constructed in a two-step infusion cloning process using pCAG-Cre (Addgene plasmid 13775, gift from C. Cepko) and pCAG-mRuby3 (Addgene plasmid 107744, gift from R. Larsen) as PCR templates to result in a Cre-mRuby fusion single ORF.
Construct:Article Title: Local circuit amplification of spatial selectivity in the hippocampus
Article Snippet: pCAG-TVA800-WT-HA was assembled by in-fusion cloning using Addgene plasmid 15778 (gift from E. Callaway). .. Notably, the HA-coding sequence was included in the primer sequences to allow for seamless HA insertion during fusion of the TVA-PCR product into the linearized XhoI/NotI pCAG vector backbone. pCAGGS-N2c(G) was a gift from T. Jessel, Addgene plasmid 73481. pCAG-GCaMP7s was assembled by in-fusion cloning using Addgene plasmid 104487 (gift from D. Kim) for PCR amplification as a template and inserted into NotI/XhoI sites of a pCAG-vector backbone according to the manufacturer’s instructions. pCAG-bReaChES-mRuby3 and pCAG-ChRmine-mScarlet were constructed through infusion cloning using pAAV-CaMKIIa-bReaChES-TS-mRuby3 and pAAV-CaMKIIa-ChRmine-TS-mScarlet as a PCR template (gifts from K. Deisseroth) into XhoI/NotI sites of a pCAG-vector backbone. .. The pCAG-Cre-mRuby fusion was constructed in a two-step infusion cloning process using pCAG-Cre (Addgene plasmid 13775, gift from C. Cepko) and pCAG-mRuby3 (Addgene plasmid 107744, gift from R. Larsen) as PCR templates to result in a Cre-mRuby fusion single ORF.
Article Title: Local circuit amplification of spatial selectivity in the hippocampus.
Article Snippet: pCAG-TVA800-WT-HA was assembled by in-fusion cloning using Addgene plasmid 15778 (gift from E. Callaway). .. Notably, the HA-coding sequence was included in the primer sequences to allow for seamless HA insertion during fusion of the TVA-PCR product into the linearized XhoI/NotI pCAG vector backbone. pCAGGS-N2c(G) was a gift from T. Jessel, Addgene plasmid 73481. pCAG-GCaMP7s was assembled by in-fusion cloning using Addgene plasmid 104487 (gift from D. Kim) for PCR amplification as a template and inserted into NotI/XhoI sites of a pCAG-vector backbone according to the manufacturer’s instructions. pCAG-bReaChES-mRuby3 and pCAG-ChRmine-mScarlet were constructed through infusion cloning using pAAV-CaMKIIa-bReaChES-TS-mRuby3 and pAAV-CaMKIIa-ChRmine-TS-mScarlet as a PCR template (gifts from K. Deisseroth) into XhoI/NotI sites of a pCAG-vector backbone. .. The pCAG-Cre-mRuby fusion was constructed in a two-step infusion cloning process using pCAG-Cre (Addgene plasmid 13775, gift from C. Cepko) and pCAG-mRuby3 (Addgene plasmid 107744, gift from R. Larsen) as PCR templates to result in a Cre-mRuby fusion single ORF.
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